Detection and Quantification of Serpula himantioides in the Wood of Chamaecyparis pisifera Butt Rot Trees by Real-Time PCR

Serpula himantioides is a wood-decaying fungal pathogen which widespread and causes potentially serious butt rot in living trees of numerous coniferous plantation species. This study aimed to quantify S. the wood Sawara cypress (Chamaecyparis pisifera) with using real-time polymerase chain reaction (real-time PCR). Species–specific primers were designed for internal transcribed spacer 2 (ITS2) regions ribosomal DNA (rDNA) himantioides. The specificity primer set was tested by end-point PCR amplicon sequencing. End-point assays positive negative lacrymans, belongs same genus as himantioides, Coniophora puteana family other decay fungi. Taxonomy assignment based on sequencing detected only spp., most them identified linearity calibration curve absolute quantification confirmed range from 101 108 copies. molecular assay method could detect trace amounts decayed wood, showing applicability early diagnostics emergence forests.